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. 2016 May 12;11(5):e0155668. doi: 10.1371/journal.pone.0155668

Fig 2. Dendritic cell migration and activation from ectocervical and endocervical tissue explants.

Fig 2

Cervical explants were exposed to cytokines (TNF-α, IL-1β, IL-8 and MIP-1β) or TLR agonists (LPS, R848, or PAM3) for 24 hours. Frequencies of DCs that had migrated out of the tissue blocks into collected culture medium, and their activation status was measured in tissue from (A) the ectocervix, and (B) the endocervix. Migration index indicates the ratio of stimulated to unstimulated DC frequencies. Activation index indicates the ratio of stimulated to unstimulated DC HLA-DR MFI. Data is presented as median with interquartile range fold induction or migration above background (medium). Each experiment was performed once and different samples were processed and stimulated for the different conditions: Medium (n = 14), cytokines (n = 14), LPS (n = 14), PAM3 (n = 14) and R848 (n = 14) for ectocervical explants; and Medium (n = 17), cytokines (n = 16), LPS (n = 17), PAM3 (n = 16) and R848 (n = 16) for endocervical explants. Wilcoxon matched-pairs signed rank test was used for statistical analyses. * P-values <0.05.