Fig 1. Fn14 expression in kidney.
A) Fn14 mRNA expression is upregulated in disease vs vehicle in the NTN model (8% and 32% vs vehicle; p<0.001). B) Immunohistochemistry Score of Fn14 in kidney tissue in NTN model. The IHC staining of kidney shows increase in Fn14 protein consistent with the extent of injury in the NTN model (p<0.005). C) Fn14 IHC reveals progressive increase in Fn14 immunopositivity in glomeruli, vessels and tubular epithelium with disease progression in NTN model. D) Linear regression graph show a positive correlation between Fn14 expression by IHC- staining with the disease severity score by H&E in NTN model (p = 0.0008), R2 = 0.914 E) Fn14 mRNA expression is increased with progression of disease in MRL/lpr mice spontaneous mouse model of Lupus Nephritis. Kidneys from MRL/lpr mice were harvested at 22 weeks of age. Aged matched controls were kidneys from MRL/MPJ mice- comparable strain that does not develop Lupus Nephritis. F) IHC showing Fn14 expression in kidney of MRL/lpr model. Fn14 expression was most prominent in arterioles and glomerulus. G) Strong association of Fn14 with inflammation score in MRL/lpr model. Linear regression plot showing correlation of Fn14 expression with inflammation (p<0.0001) and R2 = 0.63.