FIGURE 4:

Alterations in Ras activation, PIP3 production, and Rac activation in response to global chemoattractant stimulation in gflB⁻ cells. (A) WT and gflB⁻ cells expressing the indicated biosensors were uniformly stimulated with 1 μM cAMP in the presence or absence of 80 μM LY294002 or 5 μM LatA. RBD-GFP and PHcrac-RFP were coexpressed. Cells were observed by time-lapse fluorescence microscopy. Bar, 10 μm. (B) Fluorescence intensity at the cell periphery quantified relative to fluorescence intensity in the cytosol. Values represent the mean ± SEM. At least nine cells were analyzed for each group.