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GflB is required for cAMP-stimulated activation of RasG. (A) Dictyostelium cells were lysed at the indicated time points after the addition of cAMP. GST-Byr2-RBD, which binds to active RasC and RasG, was added to the lysates. The GST-fusion protein was pulled down with glutathione-Sepharose beads. The lysates and the bound fractions were analyzed by Western blotting with antibodies to RasC and RasG. (B) Quantification of the band intensities. Values represent the mean ± SEM (n = 3).
