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. 2016 May 15;27(10):1606–1620. doi: 10.1091/mbc.E15-09-0675

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© 2016 Qadota et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 10: — Overexpression of the UNC-89’s SH3 domain causes paramyosin to be mislocalized. (A) Immunolocalization of HA, paramyosin, and UNC-95 in body wall muscle cells of wild-type (no array) or transgenic animals expressing either HA-1/3IK-Ig-Fn-PK2 or HA-SH3-1/3IK-Ig-Fn-PK2 (as integrated arrays). Top row, anti-HA does not react to anything in wild-type muscle; in some animals or in some cells within the same animal, the fragments are localized to either both M-lines (arrow) and dense bodies (arrowheads) or only to M-lines (arrows). Middle row, in wild type (no array) or in animals overexpressing HA-1/3IK-Ig-Fn-PK2, paramyosin is localized to A-bands, with some reduced localization to M-lines; however, in animals overexpressing HA-SH3-1/3IK-Ig-Fn-PK2, paramyosin localizes in an abnormal pattern, to what appear to be M-lines and dense bodies. Bottom row, the overall organization of M-lines and dense bodies is normal in transgenic animals expressing either HA-1/3IK-Ig-Fn-PK2 or HA-SH3-1/3IK-Ig-Fn-PK2. (B) Coimmunostaining of anti-UNC-95 and anti-paramyosin antibodies in body wall muscle of transgenic nematodes overexpressing HA-SH3-1/3IK-Ig-Fn-PK2. UNC-95 is a marker for M-lines (arrows) and dense bodies (arrowheads). Note that paramyosin abnormally localizes to these structures. Scale bars, 10 μm.