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. 2016 May 15;27(10):1606–1620. doi: 10.1091/mbc.E15-09-0675

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© 2016 Qadota et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 9: — A small portion of UNC-89, even with an added SH3 domain, can localize to sarcomeres. (A) Top, schematic of domains within the C-terminal portion of UNC-89. Below, portions tested with HA tags in transgenic animals for localization to the sarcomere. HA-1/3IK-Ig-Fn-PK2 localized to M-lines and dense bodies. (B) Localization of transgenically expressed portions of UNC-89. Each row shows costaining with anti-HA to localize the fragment and anti-UNC-95 to mark M-lines and dense bodies. As shown, HA-Ig-Fn-PK2 does not localize, but HA-1/3IK-Ig-Fn-PK2 does. (C) Localization of transgenically expressed chimeric protein HA-SH3-1/3IK-Ig-Fn-PK2. Note that inclusion of the SH3 domain still permits 1/3IK-Ig-Fn-PK2 to localize to M-lines and dense bodies. Arrows, M-lines; arrowheads, dense bodies. Scale bar, 10 μm.