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. 2016 May 15;27(10):1650–1662. doi: 10.1091/mbc.E16-01-0033

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© 2016 Inoue and Tsai. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Grp170 overexpression stimulates NHK degradation and increases the Grp170–Se11L interaction. (A) Flp-In T-REx 293 cells or cells overexpressing WT Grp170-FLAG were transfected with NHK-HA, and the resulting WCEs were analyzed by the indicated antibodies. (B) Cells in A were treated with cycloheximide for the indicated time and harvested, and the resulting WCE was analyzed with an anti-HA antibody. (C) The NHK-HA band intensity in B was quantified with ImageJ. Data represent the mean ± SD of at least three independent experiments. (D) Flp-In T-REx 293 cells or cells overexpressing WT Grp170-FLAG were processed as in Figure 3A.