Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 May 15;27(10):1650–1662. doi: 10.1091/mbc.E16-01-0033

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Inoue and Tsai. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 7: — Grp170 promotes degradation of a nonglycosylated ERAD-Ls substrate. (A) Cells expressing TTR D18G-HA and transfected with scrambled,Grp170 siRNA (#1 or #2), or Sil1 siRNA (#1 or #2) were treated with cycloheximide for the indicated time and harvested, and the resulting WCE analyzed with an anti-HA antibody. (B, C) The TTR D18G-HA band intensity in A was quantified as in Figure 5C. (D) Top, parental Flp-In T-REx 293 cells were transfected with either scrambled or Grp170 siRNA #1. Where indicated, cells transfected with Grp170 siRNA #1 also stably expressed either siRNA-resistant WT Grp170-FLAG or G41L Grp170-FLAG. After siRNA transfection, cells were transfected with TTR D18G-HA, treated with cyclohexamide for the indicated time, and harvested, and the resulting WCE was analyzed as in A. Bottom, as for the top, except that cells stably expressing FLAG-Sil1 were used. (E) As in A, except that CD3d-YFP was used.