Extended Data Table 2. Plasmids used in this work.
Each plasmid is defined by the plasmid class, antibiotic resistance, origin of replication, and promoter/gene combinations describing the relevant ORFs carried by the plasmid. Relevant figures where these materials were used are given in each case.
| Plasmid name | Class (resistance) | Origin of replication | ORF 1 | ORF 2 | ORF 3 | Figure(s) | |||
|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||
| Promoter | Gene | Promoter | Gene | Promoter | Gene | ||||
| pAB029f | CP (specR) | ColEI | PlacZ | λcI-SH2ABL1 | PlacIq | lacI | - | - | S1A, S1C |
| pAB030g | AP (carbR) | SC101 | Plac62 | gIII, luxAB | Ptet | rpoA-HA4 | - | - | S1A |
| pAB031a | CP (specR) | ColEI | PlacZ | HA4-Zif268 | PlacIq | lacI | - | - | S1B |
| pAB035a | AP (carbR) | SC101 | Plac62 | gIII, luxAB | Ptet | rpoZ-SH2ABL1 | - | - | S1B |
| pAB035h | AP (carbR) | SC101 | Plac62 | gIII, luxAB | Ptet | rpoZ-HA4 | - | - | S1A, S1C |
| pAB042a | AP (carbR) | SC101 | Plac62 | gIII, luxAB | Ptet | rpoZ-YSX1 | - | - | S1C |
| pAB042b | AP (carbR) | SC101 | Plac62 | gIII, luxAB | Ptet | rpoZ-MBPoff7 | - | - | S1C |
| pAB042d | AP (carbR) | SC101 | Plac62 | gIII, luxAB | Ptet | rpoZ-p38_2_3 | - | - | S1C |
| pAB043a | CP (specR) | ColEI | PlacZ | λcI-MBP | PlacIq | lacI | - | - | S1C |
| pAB043c | CP (specR) | ColEI | PlacZ | λcI-p38α | PlacIq | lacI | - | - | S1C |
| pAB045a | CP (specR) | ColEI | PlacZ | rpoZ-HA4 | PlacIq | lacI | - | - | S1B, S3A, S3C |
| pAB049b | CP (specR) | ColEI | PlacZ | HA4-AsiA | PlacIq | lacI | - | - | S1A |
| pAB051e | AP (carbR) | SC101 | PExt-10consλop-62 | gIII, luxAB | Ptet | λcI-SH2ABL1 | Plpp | rpoD (R541C/F563Y/L607P) | S1A |
| pAB060c | AP (carbR) | SC101 | Plac62 | luxAB | Ppro1 | λcI-SH2ABL1 | - | - | S1B |
| pAB061i | CP (specR) | ColEI | Ppro1 | rpoZ-HA4 | PlacIq | lacI | - | - | 2A, S4B |
| pAB061i10 | CP (specR) | ColEI | Ppro1 | rpoZ-HA4 (Y87R) | PlacIq | lacI | - | - | S4B |
| pAB061i11 | CP (specR) | ColEI | Ppro1 | rpoZ-HA4 (Y87K) | PlacIq | lacI | - | - | S4B |
| pAB061i2 | CP (specR) | ColEI | Ppro1 | rpoZ-HA4 (Y87A) | PlacIq | lacI | - | - | 2A, S4B |
| pAB061i3 | CP (specR) | ColEI | Ppro1 | rpoZ | PlacIq | lacI | - | - | 2A, S4B |
| pAB061i8 | CP (specR) | ColEI | Ppro1 | rpoZ-HA4 (Y87W) | PlacIq | lacI | - | - | S4B |
| pAB061i9 | CP (specR) | ColEI | Ppro1 | rpoZ-HA4 (Y87F) | PlacIq | lacI | - | - | S4B |
| pAB064d | AP (carbR) | SC101 | Plac62 | luxAB | Ppro1 | 434cI-SH2ABL1 | - | - | S1B |
| pAB076i3 | AP (carbR) | SC101 | PlacZ-opt (OR1) | gIII, luxAB | Ppro1 | 434cI-SH2ABL1 | - | - | 2B |
| pAB076i5 | AP (carbR) | SC101 | PlacZ-opt (OR1+2+3) | gIII, luxAB | Ppro1 | 434cI-SH2ABL1 | - | - | 2B |
| pAB076i6 | AP (carbR) | SC101 | PlacZ-opt (no operator) | gIII, luxAB | Ppro1 | 434cI-SH2ABL1 | - | - | 2B |
| pAB076i7 | AP (carbR) | SC101 | PlacZ-opt (OR1) | gIII, luxAB | Ppro1 | 434cI(RR69)-SH2ABL1 | - | - | 2B |
| pAB078d10 | AP (carbR) | SC101 | PlacZ-opt (OR1) | luxAB | Ppro1 | 434cI(RR69)-SH2ABL1 | - | - | 2A, S3C |
| pAB078d3 | AP (carbR) | SC101 | PlacZ-opt (OR1) | luxAB | Ppro1 | 434cI-SH2ABL1 | - | - | 2A, S3B, S3C, S4B |
| pAB078d5 | AP (carbR) | SC101 | PlacZ-opt (OR1) | luxAB | Ppro1 | 434cI | - | - | 2A |
| pAB078d7 | AP (carbR) | SC101 | PlacZ-opt (off-target) | luxAB | Ppro1 | 434cI-SH2ABL1 | - | - | 2A |
| pAB078d8 | AP (carbR) | SC101 | PlacZ-opt (OR1+2) | luxAB | Ppro1 | 434cI-SH2ABL1 | - | - | 2A, S3C |
| pAB078d9 | AP (carbR) | SC101 | PlacZ-opt (OR1+2+3) | luxAB | Ppro1 | 434cI-SH2ABL1 | - | - | 2A |
| pAB082a | CP (specR) | ColEI | Ppro1 | rpoZ-Cry1Ac-d123 | - | - | - | S5C | |
| pAB082b | CP (specR) | ColEI | Ppro1 | rpoZ-Cry1Ac-d3 | - | - | - | S5C | |
| pAB082c | CP (specR) | ColEI | Ppro1 | rpoZ-Cry1Ac-d2A | - | - | - | S5C | |
| pAB082d | CP (specR) | ColEI | Ppro1 | rpoZ-Cry1Ac-d2B | - | - | - | S5C | |
| pAB082e | CP (specR) | ColEI | Ppro1 | rpoZ-Cry1Ac-d2C | - | - | - | S5C | |
| pAB082f | CP (specR) | ColEI | Ppro1 | rpoZ-Cry1Ac-d23A | - | - | - | S5C | |
| pAB082g | CP (specR) | ColEI | Ppro1 | rpoZ-Cry1Ac-d23B | - | - | - | S5C | |
| pAB082h | CP (specR) | ColEI | Ppro1 | rpoZ-Cry1Ac-d23C | - | - | - | S5C | |
| pAB085c | AP (carbR) | SC101 | PlacZ-opt (OR1) | luxAB | Ptet | 434cI-TnTBR3-FL | - | S5C | |
| pAB085d | AP (carbR) | SC101 | PlacZ-opt (OR1) | luxAB | Ptet | 434cI-TnTBR3-F3 | - | S5C | |
| pAB085d6 | AP (carbR) | SC101 | PlacZ-opt (OR1) | luxAB | Ppro1 | 434cI-TnTBR3-F3 | - | - | 3B |
| pAB085d7 | AP (carbR) | SC101 | PlacZ-opt (OR1) | luxAB | Ppro1 | 434cI-TnCAD-F3 | - | - | 3C |
| pAB085e | AP (carbR) | SC101 | PlacZ-opt (OR1) | luxAB | Ptet | 434cI-TnTBR3-F7 | - | S5C | |
| pAB088c | AP (carbR) | SC101 | PlacZ-opt (OR1) | gIII, luxAB | Ppro1 | 434cI-TnTBR3-F3 | - | - | 3A |
| pAB088e | AP (carbR) | SC101 | PlacZ-opt (OR1+2) | gIII, luxAB | Ppro1 | 434cI-TnTBR3-F3 | - | - | 3A |
| pAB088h | AP (carbR) | SC101 | PlacZ-opt (OR1+2) | gIII, luxAB | Ppro1 | 434cI-TnCAD-F3 | - | - | 3A |
| pAB088i | AP (carbR) | SC101 | PlacZ-opt (OR1) | gIII, luxAB | Ppro1 | 434cI(RR69)-TnCAD-F3 | - | - | 3A |
| pAB092a | AP (carbR) | SC101 | PlacZ-opt (OR1+2) | gIII, luxAB | Ppro1 | 434cI-SH2ABL1 | - | - | 2B |
| pAB094a | CP (specR) | ColEI | PBAD | rpoZ-HA4 | PC | araC | - | - | S3B |
| pAB094b | CP (specR) | ColEI | PBAD | rpoZ-HA4 (Y35A) | PC | araC | - | - | S3B |
| pAB094c | CP (specR) | ColEI | PBAD | rpoZ-HA4 (R38A) | PC | araC | - | - | S3B |
| pAB094d | CP (specR) | ColEI | PBAD | rpoZ-HA4 (E52A) | PC | araC | - | - | S3B |
| pAB094e | CP (specR) | ColEI | PBAD | rpoZ-HA4 (Y87A) | PC | araC | - | - | S3B |
| pAB107a | AP (carbR) | SC101 | PlacZ-opt (OR1) | gIII, luxAB | Ppro1 | 434cI | - | - | 2B |
| pJC175e | AP (carbR) | SC101 | PPSP | gIII, luxAB | - | - | - | - | 2B |
| SP013 | SP (kanR) | M13f1 | PgIII | rpoZ | - | - | - | S5D, S5E | |
| SP055 | SP (kanR) | M13f1 | PgIII | rpoZ-Cry1Ac | - | - | - | 3A, S5D, S5E | |
| SP096 | SP (none) | M13f1 | PgIII | rpoZ-HA4 | - | - | - | - | 2B |
| SP097 | SP (none) | M13f1 | PgIII | rpoZ-HA4 (Y87A) | - | - | - | - | 2B |
| SP098 | SP (none) | M13f1 | PgIII | rpoZ | - | - | - | - | 2B |
| MP4 | MP (chlorR) | CloDF13 | PBAD | dnaQ926, dam, seqA | PC | araC | - | - | 3A |
| MP6 | MP (chlorR) | CloDF13 | PBAD | dnaQ926, dam, seqA, emrR, ugi, cda1 | PC | araC | - | - | 3A |
| pMON101647 | EP (chlorR) | ColEI | PT7 | (none) | - | - | - | - | - |
| pMON101695 | EP (chlorR) | P15A | PT7 | MBP-TVMV | - | - | - | - | - |
| pMON133051 | EP (chlorR) | ColEI | PT7 | Cry1Ac | - | - | - | - | - |
| pMON251427 | EP (kanR) | ColEI | PT7 | TnTBR3-FL | - | - | - | - | - |
| IS0008 | EP (kanR) | ColEI | PT7 | TnCAD-FL | - | - | - | - | - |
| pMON262346 | EP (chlorR) | ColEI | PT7 | Cry1Ac | - | - | - | - | - |