Table 1.
DSC-Derived Melting Temperatures and Thermodynamic Parameters for (CTG)n and (CAG)n Stem-Loop Hairpins.1,2
| Sequence | Tm Hairpin (°C) | Tm Homoduplex (°C) | ΔH (kcal mol−1) | TΔS3 (kcal mol−1) | ΔG3 (kcal mol−1) |
|---|---|---|---|---|---|
| (CTG)6 | 65.4 | 27.8 | 22.4 ± 0.7 | 20.4 ± 0.6 | 1.9 ± 0.2 |
| (CTG)7 | 66.1 | 32.0 | 24.9 ± 2.3 | 22.8 ± 2.1 | 2.1 ± 0.2 |
| (CTG)8 | 67.3 | - | 48.1 ± 1.4 | 43.7 ± 1.2 | 4.3 ± 0.1 |
| (CTG)9 | 67.7 | 38.7 | 45.0 ± 1.0 | 40.9 ± 0.9 | 4.1± 0.1 |
| (CTG)10 | 68.2 | - | 67.7 ± 1.8 | 61.4 ± 1.6 | 6.2 ± 0.1 |
| (CTG)11 | 68.5 | 42.0 | 64.7 ± 1.2 | 58.6 ± 0.9 | 6.0 ± 0.1 |
| (CTG)12 | 68.8 | - | 86.4 ± 2.5 | 78.4 ± 2.2 | 8.0 ± 0.2 |
| (CTG)13 | 70.1 | 45.1 | 82.1 ± 1.0 | 74.1 ± 0.9 | 7.9 ± 0.1 |
| (CTG)14 | 70.0 | - | 108 ± 1 | 97.3 ± 0.6 | 10.4 ± 0.1 |
| (CAG)6 | 64.3 | - | 14.2 ± 0.6 | 13.1 ± 0.5 | 1.1 ± 0.1 |
| (CAG)7 | 64.1 | - | 15.1 ± 1.1 | 13.9 ± 1.0 | 1.2 ± 0.1 |
| (CAG)8 | 63.9 | - | 25.6 ± 0.6 | 23.5 ± 0.6 | 2.1 ± 0.1 |
| (CAG)9 | 65.2 | 16.4 | 25.0 ± 2.7 | 22.9 ± 2.4 | 2.1 ± 0.3 |
| (CAG)10 | 64.1 | - | 37.4 ± 1.1 | 34.4 ± 0.9 | 3.0 ± 0.1 |
| (CAG)11 | 64.3 | 21.6 | 38.6 ± 3.0 | 35.3 ± 2.8 | 3.2 ± 0.3 |
| (CAG)12 | 64.0 | - | 50.1 ± 1.3 | 45.9 ± 1.2 | 4.0 ± 0.1 |
| (CAG)13 | 65.0 | 24.5 | 49.8 ± 1.5 | 45.6 ± 1.2 | 4.2 ± 0.1 |
| (CAG)14 | 64.1 | - | 62.6 ± 1.4 | 57.7 ± 1.2 | 5.1 ± 0.1 |
1
In 20 mM sodium phosphate, 100 mM NaCl, pH 7.0.
2
Errors represent standard deviation from the analysis of three scans of a single sample preparation.
3
Values at 37 °C.