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. Author manuscript; available in PMC: 2017 May 12.
Published in final edited form as: J Phys Chem B. 2016 May 3;120(18):4195–4203. doi: 10.1021/acs.jpcb.6b00927

Table 2.

Thermodynamic Parameters for Conversion of (CAG)n and (CTG)n Stem-Loop Hairpins to (CAG)n/(CTG)n Duplex.1,2

n ΔH (kcal mol−1) S3 (kcal mol−1) ΔG3 (kcal mol−1)
6 −59.4 ± 3.2 −51.2 ± 2.8 −8.2 ± 0.4
7 −86.0 ± 2.8 −73.6 ± 2.5 −12.4 ± 0.3
8 −80.7 ± 2.3 −66.7 ± 2.1 −13.9 ± 0.2
9 −113 ± 4 −94.5 ± 3.2 −18.7 ± 0.5
10 −116 ± 5 −94.0 ± 3.9 −21.8 ± 1.5
11 −135 ± 3 −109 ± 3 −25.9 ± 2.2
12 −138 ± 7 −109 ± 6 −28.1 ± 1.0
13 −163 ± 5 −132 ± 5 −31.3 ± 0.7
14 −154 ± 8 −121 ± 7 −32.7 ± 1.2
1

In 20 mM sodium phosphate, 100 mM NaCl, pH 7.0.

2

Errors represent standard deviation from the analysis of three scans of a single sample preparation.

3

Values at 37 °C.