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. 2016 Jan 23;14(6):1427–1437. doi: 10.1111/pbi.12507

Figure 6.

Figure 6

(a) HIV‐1 infectivity assay. TZM‐bl cells were incubated with serial dilutions of the extracts (in triplicate) and cultured for 72 h with HIV‐1BaL using a concentration optimized for infectivity. The extent of viral replication was determined by measuring the luciferase activity of cell lysates. Ordinate: Percentage of relative infection. Abscissa: Serial dilutions. Results are shown as the percentage of infection relative to the virus‐only control ± SE. (b) Effect of different concentrations of crude extracts on cell viability. Cytotoxicity was determined using an MTT assay and was expressed as the percentage of dead cells (mean ± SD, n = 3). Ordinate: Percentage relative viability. Abscissa: Serial dilutions. WT, untransformed rice seed extract; C, negative (medium‐only) control; C+, cytotoxic positive control (nonoxynol‐9).