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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: Brain Res. 2015 Nov 12;1655:283–293. doi: 10.1016/j.brainres.2015.11.009

Figure 3.

Figure 3

Schematic demonstrating the utility of genome editing in modeling CNVs in hiPSC derived neurons. In the CRISPR-Cas9 system, the guide RNA is complexed with the Cas9 protein. When the guide RNA binds to complementary DNA, Cas9 will great a double stranded break into the DNA. Using this technology a hiPSC derived neuron from a control cell line can be engineered to possess the SZ-associated CNV or a CNV from a patient derived hiPSC derived neuron can be repaired by providing a wild type copy which can be inserted through homologous recombination. (Adapted with permission from Nestor et al, 2015).