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. 2015 Aug 17;35(18):2357–2369. doi: 10.1038/onc.2015.296

Figure 8.

Figure 8

Reconstituted ZNF322A, ADD1, CCND1 and p53 expression reverses the oncogenic effects of ZNF322A in lung cancer cells. (a and b) Knockdown of CCND1 (si-CCND1) and overexpression of p53 (over-p53) reduced cell proliferation in ZNF322A overexpressing A549 and H460 cells. (c and d) Reconstitution of ZNF322A (over-ZNF) and CCND1 (over-CCND1) and knockdown of p53 (si-p53) restored cell proliferation in ZNF322A silenced (si-ZNF) A549 and H460 cells. (e–g) Knockdown of ADD1 (si-ADD1) reversed the oncogenic effects of ZNF322A on migration and invasion abilities in vitro. (h–j) Reconstituted ZNF322A (over-ZNF) and ADD1 (over-ADD1) expression restored cell migration and invasion abilities in ZNF322A silenced lung cancer cells. Cells were transfected with indicated expression vectors with or without siRNAs. P-values determined using two-tailed Student's t-test. Data are mean±s.e.m. (n=3). **P<0.01; ***P<0.001.