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. 2016 May 13;7:650. doi: 10.3389/fmicb.2016.00650

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Copyright © 2016 Beckers, Op De Beeck, Thijs, Truyens, Weyens, Boerjan and Vangronsveld.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of parametrical alpha diversity between selected 16S rDNA primer pairs (799F -1391R, 799F-1193R, and 341F-783Rabc) for all sampled plant compartments [(A) Rhizosphere soil, (B) Root, (C) Stem, (D) Leaf] after subsampling to 417 reads. All averages were calculated across three biologically independent poplar individuals for each primer pair. Left panels: average number of operational taxonomic units (OTUs) observed based on a 97% sequence similarity cutoff (richness) and right panels: Inverse Simpson diversity indices. OTU counts and Inverse Simpson indices were statistically analyzed using a one-way ANOVA per plant compartment. Differences at the 95% significance level are indicated with lower case letters (P < 0.05). nd = not determined as a result of low bacterial rDNA reads.