Table 1.

Summary of primers used in the current study.

Primer pairs	Primer sequence (5′−3′)	A	B	C	D	References
799F	AACMGGATTAGATACCCKG	79.7	0.29	4	V5-V6-V7	Chelius and Triplett, 2001
1391R	GACGGGCGGTGWGTRCA	84.6	1.44	0		Walker and Pace, 2007
967F	CAACGCGAAGAACCTTACC	80.9	0.34	0	V6-V7	Sogin et al., 2006
1391R	GACGGGCGGTGWGTRCA	84.6	1.44	0		Walker and Pace, 2007
799F	AACMGGATTAGATACCCKG	79.7	0.29	4	V5-V6-V7	Chelius and Triplett, 2001
1193R	ACGTCATCCCCACCTTCC	78.1	0.20	0		Bodenhausen et al., 2013
341F	CCTACGGGNGGCWGCAG	91.2	0.05	0	V3-V4	Klindworth et al., 2013
785R	GACTACHVGGGTATCT AATCC	86.2	0.09	0		Klindworth et al., 2013
68F	TNANACATGCAAGTCGRRCG	72.5	0.60	0	V1-V4	McAllister et al., 2011
783Rabc	CTACC*AGGGTATCTAATCC*TG	70.9	5.05	3		Sakai et al., 2004
68F	TNANACATGCAAGTCGRRCG	72.5	0.60	0	V1-V3	McAllister et al., 2011
518R	WTTACCGCGGCTGCTG G	87.6	0.09	0		Lee et al., 2010
341F	CCTACGGGNGGCWGCAG	91.2	0.05	0	V3-V4	Klindworth et al., 2013
783Rabc	CTACC*AGGGTATCTAATCC*TG	70.9	5.05	3		Sakai et al., 2004

Primers are indicated as forward (F) or reverse (R).

^*Primer 783Rabc is a primer mix (Sakai et al., 2004):

(a) 5′-CTACCAGGGTATCTAATCCTG-3′,

(b) 5′-CTACCGGGGTATCTAATCCCG-3′,

(c) 5′- CTACCCGGGTATCTAATCCGG-3′.

(A) Primer coverage (%) for Bacteria using Silva (Quast et al., 2013) and probeBase (Loy et al., 2007), (B) Primer Score tested with PrimerProspector 1.0.1 (Walters et al., 2011) (Table S1), (C) Number of mismatches with poplar chloroplast DNA, (D) Hypervariable region of the 16S rRNA operon targeted by primer pairs.