Table 1B.
Characteristic properties of A. malaysianum cultures: Mycelia from exponential phase of A. malaysianum (approximately after 24 h) were washed and transferred into either glucose containing 2-deoxy D-glucose or cellobiose containing 2-deoxyglucose or in minimal medium containing just cellobiose.
Property | Glucose +2-DG | Cellobiose +2-DG | Cellobiose control |
---|---|---|---|
Carbon content(g/l) | 10±0.001 | 10±0.001 | 10±0.001 |
β-glucosidase (U/ml) | 2±0.3 | 14±1.23 | 4±0.5 |
β,1,3 glucanase (U/ml) | 0.2±0.02 | 1.78±0.05 | 0.5±0.23 |
Radical scavenging activity | 2.5±0.001 | 8.1±0.001 | 3.5±0.11 |
Superoxide content | 0.025±0.01 | 0.066±0.01 | 0.047±0.35 |
Change in Dry Cell Mass (g/l) | 0.5±0.33 | 1.5±0.5 | 3.0±1.2 |
Ph | 5±0.001 | 4.5±0.002 | 5±0.01 |
Chitinase (U/ml) | 0.4±0.2 | 1.75±0.7 | 0.39±0.43 |
Endoglucanase (U/ml) | 0.22±0.01 | 4±0.22 | 1.3±0.1 |
Hyphae fragmentation | no | yes | no |
Pigment formation | no | yes | no |
Mean ± SD values calculated from five independent experiments are presented.