Table 2.

Reads pre-processing and transcript assembly statistics using Trinity.

Library	Total Reads#	High quality reads#	Equalized high quality reads#	Number of assembled Transcripts	Corresponding Number of genes	N50, Average transcript length, GC content of final assembly
Control	25329795 (R) +	22273695 (R) +	20744877 (R) +	27252	21192	2414, 1296.79,
	25329795 (F)	22737139 (F)	20744877 (F)			54.13%
		80%	70%
Treated	38573096 (R) +	36395969 (R) +	34885064 (R) +	32982	22372	2770, 1580.90,
	38573096 (F)	36329694 (F)	34885064 (F)			53.8%
		80%	70%
Merged	63902891 (R) +	55629941 (R) +	55629941 (R) +	36248	22847	3659, 2055.82,
	63902891 (F)	55629941 (F)	55629941 (F)			53.5%
		80%	80%

^#Total reads indicate the number of raw reads. High quality reads are the quality filtered reads obtained from the raw reads. Equalized reads are the pairs of high quality reads available. Since in many cases, only one of the pair is good quality where as the other pair is poor quality. In that case, the pair of reads are removed.