Table 1.

Response of TREK-2 tandem-linked channels and mutants to 2-APB and pH_o changes.

	2-APB response		pHo response
Channel	ARamax	nb	IRcmax	nb
WT-WT	18.13 ± 1.30	8–13	0.73 ± 0.02	8–9
ΔpCt-ΔpCt	4.34 ± 0.10	7	0.46 ± 0.01	7
WT-ΔpCt	15.50 ± 0.74	5–7	0.79 ± 0.01	5
ΔpCt-WT	17.37 ± 1.56	5–6	0.77 ± 0.02	6
WT-ΔCt	18.15 ± 2.42	5–7	0.76 ± 0.03	7–9
ΔCt-WT	16.78 ± 1.50	6–7	0.81 ± 0.02	6–10
WT-G312A	17.16 ± 1.00	6–8	0.76 ± 0.04	6–7
G312A-WT	16.84 ± 2.00	6–7	0.79 ± 0.01	6–7
G312A-G312A	7.80 ± 0.50	5–7	0.23 ± 0.02	5–7
G312A-ΔpCt	5.32 ± 0.46	7	0.43 ± 0.03	11–12
G312A-G312A/ΔpCt	3.36 ± 0.37	7–8	0.12 ± 0.05	7–12

^aThe maximal concentrations of 2-APB used in these curves is 333 μM for these channels, except for ΔpCt-ΔpCt (800 μM). ^bThe number of oocytes used for each point in concentration response curves. ^cThe maximal inhibition produced by pH9.3.