Figure 4.

C3a-Based Chemotaxis between Cells of the Deep and Superficial Layers

(A–K) Testing the basic assumption of the model. Classical chemotaxis assay using DL and SL explants (A), or DL explants with purified C3a protein (G). Trajectories of SL (B) and DL (C–F, H, and I) explants, and DL chemotaxis toward non-functional (C3aDesArg, H, n = 35) and functional (I, n = 38) C3a source with corresponding chemotaxis indices in vitro (J) and in silico (K). Error bars: SD. See also Figure S6.