Figure 7.

Dorsoventral Differences in Expansion Explained by Differential C3 Expression

(A) The extent of thinning and expansion depends on the level of chemoattractant in the SL in silico.

(B) Tissue expansion and thickness shown after 4 hr in simulations as a function of chemoattractant levels.

(C and D) Approximation of C3 levels in vivo using intensity levels from ISH of a stage 10 embryo reveals differential expression in the animal cap with a reduced level in the ventral region (66% compared with the whole-animal cap) and an increased level in the dorsal region (172%).

(E and F) Expansion in vivo in the ventral and dorsal areas of the animal pole. Expansion in the dorsal regions was significantly higher (n = 20) than in the ventral regions, as predicted by the model. Error bars in (B) and (F) show the SD; error bars in (D) show uncertainty of sampling. Significance ^∗∗∗p < 0.001, ^∗p < 0.05.