Figure 8.

Localization of Chemoattractant Source Determines Radial Intercalation

(A–H) Simulated cell configurations and corresponding chemoattractant levels at the end of epiboly with different localizations of chemoattractant production: only in the SL (Control, A, E), nowhere (Inhibition, B, F), in every cell (Ubiquitous, C, G), and in a restricted region of the SL (Localized, D, H, producing region shown in red).

(I–L) Experimental validation of the predicted behavior showing sections of the blastocoel roof of stage 11 embryos. Red, nucleus; green, membrane; graft in (L) marked with cytosolic RLDx. White bar indicates tissue thickness.

(M) Quantification of tissue thickness, measured in units of cell diameter after normal epiboly (20 μm), for the four settings in silico and in vivo. Error, SD. Significances compared with relevant control: ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001; ns, p > 0.05. 1.3× Chem denotes 1.3-fold overexpression in both DCs and SCs.