Figure 2. Intracellular Zn²⁺‐induced Kv2.1 channel dispersal is calcineurin‐dependent .

A, confocal images of cortical neurons transfected with a GFP‐tagged Kv2.1 construct, representative of Kv2.1 localization following treatment with either ZnPyr (30 μm ZnCl₂, 300 nm pyrithione) or ZnPyr + FK520 (30 μm ZnCl₂, 300 nm pyrithione, 5 μm FK520) conditions for 2 h. Control images not relevant to this finding were omitted; both vehicle‐treated and FK520‐treated neurons showed significant Kv2.1 channel clustering. Scale bar = 10 μm. B, showing the mean number of Kv2.1 clusters found in each condition, normalized to neuronal somatic area (number Kv2.1 clusters μm^–2 of neuronal soma). Data are shown for each condition as the mean ± SEM (Vehicle, 0.141 ± 0.014, n = 20; ZnPyr 2 h, 0.072 ± 0.008, n = 41; ZnPyr + FK520 2 h, 0.168 ± 0.014, n = 24; FK520 2 h, 0.147 ± 0.020 Kv2.1, n = 9). Analysed via one‐way ANOVA with Dunnett's MCT vs. vehicle‐treated neurons (***P < 0.001).