Table 1.
Precision studies for LC-MS/MS and two immunoassays performed using same two serum pools with different 25(OH)D concentrations
| Within-run | Between-run | |||||
|---|---|---|---|---|---|---|
| Mean ± SD (nmol/L) | CV (%) | Mean ± SD (nmol/L) | CV (%) | |||
| Centaur | pool 1 | 132.0 ± 13.6 | 10.3 | 130.4 ± 14.3 | 11 | |
| pool 2 | 338.0 ± 31.1 | 9.2 | 340.2 ± 17.3 | 5.1 | ||
| Elecsys | pool 1 | 14.0 ± 0.2 | 1.4 | 14.3 ± 3.6 | 25.2 | |
| pool 2 | 33.6 ± 0.5 | 1.5 | 36.2 ± 7.1 | 19.6 | ||
| LC-MS/MS 25(OH)D2 | pool 1 | 18.7 ± 0.5 | 2.7 | 19.3 ± 1.1 | 5.7 | |
| pool 2 | 44.4 ± 1.0 | 2.3 | 46.5 ± 2.4 | 5.2 | ||
| LC-MS/MS 25(OH)D3 | pool 1 | 17.9 ± 0.4 | 2.2 | 18.3 ± 0.8 | 4.4 | |
| pool 2 | 38.9 ± 1.3 | 3.3 | 37.9 ± 1.7 | 4.5 | ||