Fig. 1.

Measurement of proteolytic activity of Bar-I. (A) Fibrinolytic activity of Bar-I. The fibrinolytic activity was measured in triplicates by the halo size on a fibrin bed following overnight incubation at 37 °C with several concentrations of Bar-I, crude venom of B. barnetti and plasmin (0.5–2.0 μg/μl). The data are expressed as means of triplicate assays with standard errors. Effect of pH (B) and temperature (C) on the proteolytic activity of Bar-I using DMC as substrate. Each point represent the mean and standard error of three individual experiments.