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. 2016 May 13;6:25893. doi: 10.1038/srep25893

Figure 4. Shear stress induces osteopontin expression in vascular endothelial cells via P2X4 receptor signaling.

Figure 4

(a) Fluid stimulation (48 hr, 15 dyne/cm2 shear stress) upregulated the mRNA expression of osteopontin in b.End3 cells (p = 0.0314, *p < 0.05, Student’s t-test). The data represent the mean ± s.e.m. (n = 3 each). (b) mRNA expression of the P2X4 receptor in b.End3 cells was not affected by fluid stimulation (48 hr, 15 dyne/cm2 shear stress, p = 0.9237 Student’s t-test). The data represent the mean ± s.e.m. (n = 6 each). (c) mRNA expression of the P2X4 receptor in b.End3 cells was downregulated 72 hr after P2X4 receptor siRNA transfection (p = 0.0341, *p < 0.05, Student’s t-test). The data represent the mean ± s.e.m. (n = 4 each). (d) Upregulation of osteopontin mRNA in b.End3 cells by fluid stimulation (48 hr, 15 dyne/cm2 shear stress) was inhibited by P2X4 receptor siRNA (p = 0.0358, *p < 0.05, ANOVA with Tukey-Kramer test). Cells had previously been transfected with control siRNA or P2X4 receptor siRNA 3 days before fluid stimulation. The data represent the mean ± s.e.m. (Control group, Control siRNA, n = 6; P2X4 receptor siRNA n = 3; Shear stress group, Control siRNA, n = 6; P2X4 receptor siRNA, n = 7). (e) mRNA expression of osteopontin in b.End3 cells was upregulated 48 hr after ivermectin treatment (3 μM, p = 0.0003, **p < 0.01, ANOVA with Tukey-Kramer test). The data represent the mean ± s.e.m. (n = 3 each).