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. 2016 May 13;6:25870. doi: 10.1038/srep25870

Figure 1. The expression of CNTF is up-regulated after injury.

Figure 1

The cultured TKE2 cells were stained with CNTF (a), compared to negative control (b). (c) Gene expression of Cntf at post-injury 0 h, 1 h, 3 h, 6 h, 12 h, 24 h and 36 h was compared. The levels of Cntf are normalized to the 0 h time point. ‘h’ indicates hours. (d,e) Protein was extracted and evaluated at 0 h, 6 h, 12 h, 24 h, 36 h and 48 h after scratch. The levels of CNTF are normalized to the 0h time point. ‘h’ indicates hours. (f–h) The injury area of cultured cells was stained with CNTF at 16 h post scratching. h is the merged pictures of (f,g). Dash line indicates the edge of scratch caused by the tip. n = 3 per group. The experiments were performed three times with similar results. Statistical analysis was performed using One-way ANOVA and Bonferroni post hoc test. *Significant difference between two time-points at p < 0.05; **Significant difference between two time-points at p < 0.01.