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. 2016 May 13;6:25870. doi: 10.1038/srep25870

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Copyright © 2016, Macmillan Publishers Limited

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(a) The mRNA level of Mmp3 and Mmp14 was evaluated by qPCR after Akt inhibitor treatment together with CNTF as compared to CNTF alone. Akti, Akt inhibitor treatment. (b) The protein level of MMP3 and MMP14 was evaluated by Western blot. (c,d) The expression of MMP3 and MMP14 after Akt inhibitor treatment was evaluated by immunofluorescence staining. The right column is the merged picture of left column and middle column. n = 3 per group. The experiments were performed three times with similar results. Statistical analysis was performed using Student’s t-test. *Significant difference between two groups at p < 0.05. **Significant difference between two groups at p < 0.01.