Table 1.
ARP-HNE modified peptide list from the current study.
| Protein name | Gene name | Uniprot ID | MW | Sequence | Adducted residue |
|---|---|---|---|---|---|
| ATP synthase subunit beta, mitochondrial | atp5b | ATPB_RAT | 56 kDa | (R)LVLEVAQhLGES TVR(T) | His |
| (K)AhGGYSVFAGVGER(T) | His | ||||
| (R)TREGNDLYhEMIESGVINLK(D) | His | ||||
| (R)EGNDLYhEMIESGVINLK(D) | His | ||||
| (K)KGSITSVQAIYVPADDLTDPAPATTFAhLDATTVLSR(A) | His | ||||
| (R)IMDPNIVGSEhYDVAR(G) | His | ||||
| (R)FLSQPFQVAEVFTGHMGk(L) | His | ||||
| (K)GFQQILAGDYDhLPEQAFYmVGPIEEAVAK(A) | His | ||||
| (K)LAEEhGS(−) | His | ||||
| D-beta-hydroxybutyrate dehydrogenase, mitochondrial | Bdh1 | BDH_RAT | 38 kDa | (K)AVLVTGcDSGFGFSLAK(H) | Cys |
| (R)TIQLNVcNSEEVEK(A) | Cys | ||||
| (K)mETYcNSGSTDTSSVINAVTHALTAATPYTR(Y) | Cys | ||||
| Voltage-dependent anion-selective channel protein 1 | Vdac1 | VDAC1_RAT | 31 kDa | (K)YQVDPDAcFSAK(V) | Cys |
| (K)LTLSALLDGKNVNAGGHk(L) | Lys | ||||
| (K)NVNAGGhK(L) | His | ||||
| ADP/ATP translocase 2 | Slc25a5 | ADT2_RAT | 33 kDa | (K)LLLQVQhASK(Q) | His |
| (K)GTDImYTGTLDcWR(K) | Cys | ||||
| Long-chain-fatty-acid—CoA ligase 1 | Acsl1 | ACSL1_RAT | 78 kDa | (K)ALkPPCDLSmQSVEVTGTTEGVR(R) | Lys |
| (R)GIQVSNDGPcLGSR(K) | Cys | ||||
| (K)GIAVhPELFSIDNGLLTPTLK(A) | His | ||||
| Carbamoyl-phosphate synthase [ammonia], mitochondrial | Cps1 | CPSM_RAT | 165 kDa | (R)SAYALGGLGSGIcPNK(E) | Cys |
| (R)SAYALGGLGSGIcPNKETLMDLGTK(A) | Cys | ||||
| (R)VSQEhPVVLTK(F) | His | ||||
| (R)FLGVAEQLhNEGFK(L) | His | ||||
| Uricase | Uox | URIC_RAT | 35 kDa | (K)DYLhGDNSDIIPTDTIK(N) | His |
| (K)NTVhVLAK(F) | His | ||||
| 60 kDa heat shock protein, mitochondrial | Hspd1 | CH60_RAT | 61 kDa | (K)ISSVQSIVPALEIANAhR(K) | His |
| (R)AAVEEGIVLGGGcALLR(C) | Cys | ||||
| Microsomal glutathione S-transferase | Mgst1 | B6DYQ4_RAT | 17 kDa | (K)VFANPEDcAGFGK(G) | Cys |
| (R)IYhTIAYLTPLPQPNR(G) | His | ||||
| Catalase | Cat | CATA_RAT | 60 kDa | (K)NAIHTYVQAGShIAAK(G) | His |
| (R)LGPNYLQIPVNcPYR(A) | Cys | ||||
| (R)GPLLVQDVVFTDEMAhFDR(E) | His | ||||
| (R)DAMLFPSFIhSQK(R) | His | ||||
| Choline dehydrogenase (Fragment) | Chdh | Q64644_RAT | 49 kDa | (R)KPTQQEAYQVhVGTMR(A) | His |
| (K)hELGANMYR(G) | His | ||||
| (K)GcPALGDENVPVYKPQTLDTQR(-) | Cys | ||||
| ATP synthase subunit gamma, mitochondrial | Atp5c1 | ATPG_RAT | 30 kDa | (R)ThSDQFLVSFK(D) | His |
| (K)hLIIGVSSDR(G) | His | ||||
| 3-ketoacyl-CoA thiolase, mitochondrial | Acaa2 | THIM_RAT | 42 kDa | (K)TNVSGGAIALGhPLGGSGSR(I) | His |
| (K)LEDTLWAGLTDQHVk(L) | Lys | ||||
| Mitochondrial carrier homolog 2 | Mtch2 | B0BN52_RAT | 34 kDa | (K)VLQYYQEcEKPEDLGSANVQK(E) | Cys |
| ATP synthase subunit alpha, mitochondrial | Atp5a1 | ATPA_RAT | 60 kDa | (K)hALIIYDDLSK(Q) | His |
| ATP synthase subunit b, mitochondrial | Atp5f1 | AT5F1_RAT | 29 kDa | (R)LDYhISVQDMmR(R) | His |
| (R)hYLFDVQR(N) | His | ||||
| (K)hVIQSISAQQEK(E) | His | ||||
| Prohibitin-2 | Phb2 | PHB2_RAT | 33 kDa | (R)IGGVQQDTILAEGLhFR(I) | His |
| Voltage-dependent anion-selective channel protein 3 | Vdac3 | VDAC3_RAT | 31 kDa | (K)ScSGVEFSTSGHAYTDTGK(A) | Cys |
| (K)NFNAGGhK(V) | His | ||||
| Sideroflexin-1 | Sfxn1 | SFXN1_RAT | 36 kDa | (K)hVSPLIGR(F) | His |
| Glutamate dehydrogenase 1, mitochondrial | Glud1 | DHE3_RAT | 61 kDa | (K)hGGTIPVVPTAEFQDR(I) | His |
| ATP synthase subunit d, mitochondrial | Atp5fh | ATP5H_RAT | 19 kDa | (K)NcAQFVTGSQAR(V) | Cys |
| Cytochrome b-c1 complex subunit 2, mitochondrial | Uqcrc2 | QCR2_RAT | 48 kDa | (R)YENYNYLGTShLLR(L) | His |
| (K)NALANPLYcPDYR(M) | Cys | ||||
| Cytochrome c oxidase subunit 4 isoform 1, mitochondrial | Cox4i1 | COX41_RAT | 20 kDa | (K)SYVYGPIPhTFDR(D) | His |
| (R)DYPLPDVAhVK(L) | His | ||||
| Bile acid-CoA:amino acid N-acyltransferase | Baat | BAAT_RAT | 46 kDa | (K)LTAVPLSALVDEPVhIR(V) | His |
| Mitochondrial carnitine/acylcarnitine carrier protein | Slc25a20 | MCAT_RAT | 33 kDa | (K)SVhDLSVPR(V) | His |
| (R)LQTQPPSLPGQPPMYSGTIDCFRk(T) | Lys | ||||
| NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial | Ndufa10 | NDUAA_RAT | 40 kDa | (R)VITVDGNIcSGK(N) | Cys |
| Nicotinamide nucleotide transhydrogenase | Nnt | Q5BJZ3_RAT | 114 kDa | (R)EANSIVITPGYGLcAAK(A) | Cys |
| Voltage-dependent anion-selective channel protein 2 | Vdac2 | VDAC2_RAT | 32 kDa | (K)SFNAGGhK(L) | His |
| (K)ScSGVEFSTSGSSNTDTGK(V) | Cys | ||||
| Peroxisomal acyl-coenzyme A oxidase 2 | Acox2 | ACOX2_RAT | 77 kDa | (R)SLEDhTPLPGITVGDIGPK(M) | His |
| Mitochondrial dicarboxylate carrier | Slc25a10 | O89035_RAT | 31 kDa | (R)GALVTVGQLScYDQAK(Q) | Cys |
| Pyruvate carboxylase, mitochondrial | Pc | PYC_RAT | 130 kDa | (R)LDNASAFQGAVISPHYDSLLVk(V) | Lys |
| Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial | Aldh6a1 | MMSA_RAT | 58 kDa | (K)GYENGNFVGPTIISNVKPSMTCYk(E) | Lys |
| Aldehyde dehydrogenase, mitochondrial | Aldh2 | ALDH2_RAT | 56 kDa | (K)VAFTGSTEVGhLIQVAAGSSNLK(R) | His |
| Aspartate aminotransferase, mitochondrial | Got2 | AATM_RAT | 47 kDa | (K)TcGFDFSGALEDISK(I) | Cys |
| 3 beta-hydroxysteroid dehydrogenase type 5 | Hsd3b5 | 3BHS5_RAT | 42 kDa | (K)SQSIQGQFYYISDDTPhQSYDDLNYTLSK(E) | His |
| Very long-chain specific acyl-CoA dehydrogenase, mitochondrial | Acadvl | ACADV_RAT | 71 kDa | (R)TGIGSGLSLSGIVhPELSR(S) | His |
| Ndufa9 protein | Ndufa9 | B5DER7_RAT | 43 kDa | (K)AVQhSNVVINLIGR(E) | His |
| Trifunctional enzyme subunit beta, mitochondrial | Hadhb | ECHB_RAT | 51 kDa | (R)LNFLSPELPAVAEFSTNETMGhSADR(L) | His |
| Tricarboxylate transport protein, mitochondrial | Slc25a1 | TXTP_RAT | 34 kDa | (K)FIhDQTSSNPK(Y) | His |
| Succinate dehydrogenase complex, subunit C, integral membrane protein | Sdhc | Q641Z9_RAT | 18 kDa | (K)NTSSNRPVSPhLTIYR(W) | His |
| CDGSH iron-sulfur domain-containing protein 1 | Cisd1 | CISD1_RAT | 12 kDa | (K)hNEETGDNVGPLIIK(K) | His |
| Fatty aldehyde dehydrogenase | Aldh3a2 | AL3A2_RAT | 54 kDa | (R)FDhILYTGNTAVGK(I) | His |