Table 2.
The combined 31 proteins identified in both protein- and peptide-level enrichment workflows.
| Protein name | Gene name | Occurrence of ArpHNE peptides | Reactivity category | Correlation coefficient | Regression coefficient |
|---|---|---|---|---|---|
| ATP synthase subunit beta, mitochondrial | Atp5b | 38 | A | 0.627 | 110812 |
| D-beta-hydroxybutyrate dehydrogenase, mitochondrial | Bdh1 | 21 | A | 0.764 | 27361 |
| Carbamoyl-phosphate synthase [ammonia], mitochondrial | Cps1 | 12 | A | 0.729 | 68122 |
| Long-chain-fatty-acid—CoA ligase 1 | Acsl1 | 12 | A | 0.352 | 6051 |
| Voltage-dependent anion-selective channel protein 1 | Vdac1 | 11 | A | 0.635 | 311 |
| Microsomal glutathione S-transferase | Mgst1 | 9 | B | 0.775 | 3656 |
| ADP/ATP translocase 2 | Slc25a5 | 8 | B | 0.981 | 2993 |
| 3-ketoacyl-CoA thiolase, mitochondrial | Acaa2 | 7 | C | 0.982 | 2879 |
| ATP synthase subunit gamma, mitochondrial | Atp5c1 | 6 | B | 0.985 | 521 |
| Catalase | Cat | 6 | A | 0.636 | 40051 |
| Glutamate dehydrogenase 1, mitochondrial | Glud1 | 5 | B | 0.908 | 14995 |
| Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial | Etfdh | 5 | B | 0.899 | 2470 |
| ATP synthase subunit alpha, mitochondrial | Atp5a1 | 4 | B | 0.910 | 29111 |
| Prohibitin-2 | Phb2 | 4 | B | 0.998 | 4138 |
| Choline dehydrogenase (Fragment) | Chdh | 4 | other | 0.406 | 7508 |
| ATP synthase subunit d, mitochondrial | Atp5h | 4 | B | 0.917 | 65516 |
| Trifunctional enzyme subunit beta, mitochondrial | Hadhb | 3 | A | 0.845 | 1507 |
| Bile acid-CoA:amino acid N-acyltransferase | Baat | 3 | A | 0.277 | 1366 |
| Sideroflexin-1 | Sfxn1 | 3 | B | 0.989 | 3523 |
| Cytochrome c oxidase subunit 4 isoform 1, mitochondrial | Cox4i1 | 3 | C | 0.982 | 1197 |
| 60 kDa heat shock protein, mitochondrial | Hspd1 | 2 | A | 0.553 | 7760 |
| CDGSH iron-sulfur domain-containing protein 1 | Cisd1 | 2 | C | 0.982 | 285 |
| Aldehyde dehydrogenase, mitochondrial | Aldh2 | 2 | other | 0.107 | 3837 |
| Voltage-dependent anion-selective channel protein 2 | Vdac2 | 2 | A | 0.217 | 182 |
| Hydroxymethylglutaryl-CoA synthase, mitochondrial | Hmgcs2 | 2 | A | 0.382 | 2750 |
| Nicotinamide nucleotide transhydrogenase | Nnt | 2 | A | 0.542 | 9062 |
| Cytochrome b-c1 complex subunit 2, mitochondrial | Uqcrc2 | 2 | A | 0.617 | 1827 |
| Mitochondrial dicarboxylate carrier | Slc25a10 | 2 | B | 0.928 | 2482 |
| ATP synthase subunit b, mitochondrial | Atp5f1 | 1 | C | 0.985 | 1774 |
| Aspartate aminotransferase, mitochondrial | Got2 | 1 | N/A | N/A | 0 |
| Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial | Sdha | 1 | other | −0.523 | −1758.474 |
The list is sorted by total occurrence of ARP-HNE sites. Linear regression and correlation were applied to evaluate the concentration-dependency of protein abundance on HNE concentration; the regression coefficient represents the slope of the regression line and the correlation coefficient represent the linearity of the correlation between adduct accumulation and HNE concentration.