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. 2016 May 9;7:11557. doi: 10.1038/ncomms11557

Figure 2. SHG-specificity of Ap3.

Figure 2

(a) Representative SHG and TPF signals obtained from cultured CHO cells loaded with 20 μM FM4-64 (left) and Ap3 (right). The signal intensity is shown in pseudocolour. Scale bar, 20 μm. (b) Quantitative analysis of SHG and TPF signals. Signals of SHG (left) and TPF (middle) from the plasma membranes of CHO cells stained with Ap3 (red) and FM4-64 (black) were quantified and summarized. Individual points indicate data from each cell (n=27 cells for Ap3 and 28 cells for FM4-64) and boxes indicates the 25th, 50th and 75th percentile values.