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. 2016 May 13;6:25885. doi: 10.1038/srep25885

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Copyright © 2016, Macmillan Publishers Limited

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(a) Uptake of S. japonicum EVs by normal mouse liver cells detected using fluorescence microscopy. Approximately 10 μg of PKH67-labelled S. japonicum EVs, NCTC clone 1469 exosomes, and a PKH67-PBS control were added to the cells. Uptake of the labeled exosomes by NCTC clone 1469 cells was detected using fluorescence microscopy. Nuclei were stained with DAPI (blue). (b) qRT-PCR analysis of S. japonicum EV associated miRNAs in treated cells. Results represent mean ± SEM from triplicate experiments. *P ≤ 0.05 and **P ≤ 0.01 (Tukey’s test, one-way ANOVA).