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. 2016 May 13;6:25838. doi: 10.1038/srep25838

Figure 3. In vitro differentiation potential of pESL cells.

Figure 3

(a) EB derived from pESLCs at passage 19 by hanging drop culture on no adhesive culture dishes for 10 days. (b) EB spread on dishes coated with gelatin and displayed distinct signs of differentiation. (c) Expression of the β-III TUBULIN (endoderm), ENOLASE (mesoderm), and AMYLASE (ectoderm) were detected in EB resulted from pESLCs at passage 2 and passage 19. Brain, liver and muscle tissues of Landrace pig were used as positive control. (d) Expression of differentiation marker Cytokeratin 17 (endoderm), Desmin (mesoderm) and Neurofilament (ectoderm) from differentiated pESL cells were confirmed by the immunocytochemistry analysis. Scale bar = 100 um.