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. 2016 May 11;7:11445. doi: 10.1038/ncomms11445

Figure 2. Nine weeks of CR prevents cerebellar neuropathology in Tg MJD mice.

Figure 2

(a) Cresyl violet-stained sections demonstrate the thickness of molecular layer (ML) and granular layer (GL) of lobule V of cerebellum in 15-week-old littermate WT and Tg MJD mice in AL or CR diet, quantified in b and c. Scale bar, 50 μm. (d) Quantification of cerebellar volume showed a significantly higher cerebellar volume in calorie-restricted mice. (e) Analysis of the number of mutant ataxin-3 inclusions in the cerebellar Purkinje cells of Tg MJD mice. The total number of aggregates in Purkinje cells is significantly lower in calorie-restricted mice, in comparison with AL mice. (f) Relative levels of the soluble form of mutant ataxin-3 normalized with β-actin were significantly decreased in Tg mice in the CR diet. Data represent mean±s.e.m.; NS P>0.05 and *P<0.05 compared with Tg-AL. (b–d). One-way analysis of variance with Bonferroni's post-hoc test. (e,f) Unpaired Student's t-test. WT-AL n=3; WT-CR n=4; Tg-AL n=4; Tg-CR n=4.