FIGURE 3.

Methylation pattern of mIno1 DNA is altered in IP6K1-KO cells. A, MEF cells were grown in DMEM with 10% FBS and harvested for DNA isolation. DNA methylation levels were determined as described under “Experimental Procedures.” CpG islands are depicted as balloons. Methylated cytosines are filled, and unmethylated cytosines are unfilled. B, raw data of mIno1 DNA methylation experiment. CpG islands are depicted in bold. Methylated cytosines are depicted in red, and unmethylated cytosines are depicted in black. CpG islands with altered methylation in IP6K1-KO cells are highlighted with yellow shading, and the start codon is highlighted with green shading.