TABLE 4.

Measurement of cisplatin-induced CD44v3^highALDH1^high cell growth inhibition

* Tumor cell growth inhibition (IC₅₀) is designated as “the micromolar concentration of chemotherapeutic drug (e.g. cisplatin, 24h treatment) that causes 50% inhibition of tumor cell growth” using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-based growth assay as described under “Experimental Procedures.” IC₅₀ values are presented as the means ± S.E. (with n = 4). All assays consisted of at least six replicates and were performed on at least 4 different experiments.

^a Data are statistically significant (P < 0.001; analysis of variance; n = 4) as compared with control samples (normal IgG-treated cells without HA addition).

^b,d Data are statistically significant (P < 0.001; analysis of variance; n = 4) as compared with control samples (scrambled siRNA-treated cells without HA addition).

^c Data are statistically significant (P < 0.001; analysis of variance; n = 4) as compared with control samples (miRNA negative control-treated cells without HA addition).