TABLE 4.

Peptide spectral counts for identified N-glycopeptides with varying N-glycan lengths

CsCl gradient fractions 4 and 5 from the WT were analyzed by MS applying HCD fragmentation. N-Glycopeptides with N-glycans ranging from one to five carbohydrate residues (depicted as symbols: hexose (○), hexuronic acid (♢), methylated hexuronic acid (♢^Me), as well as non-glycosylated peptides (P)) were identified in the given number of spectra. Peptide spectral counts from fractions 4 and 5 were combined. TSPQASFDFDYTN@TSGN@LTITHE from PilA2 was not included as its two N-glycosites can harbor different glycans (see Fig. 5) (n = 1).