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. 2016 Mar 24;291(20):10625–10634. doi: 10.1074/jbc.M116.713768

FIGURE 4.

FIGURE 4.

Suppression of miR-291b-3p expression ameliorates oleic acid/palmitic acid mixture-induced lipid accumulation in NCTC1469 cells. A–E, NCTC1469 cells were treated with the mixture of oleic acid/palmitic acid (2:1, m (mol/liter)/m (mol/liter)] for 24 h in DMEM containing 10% horse serum, followed by transfection with a miR-291b-3p inhibitor or NC for 48 h in the presence of oleic acid/palmitic acid. A and B, real-time PCR analysis of miR-291b-3p expression. C, oil Red O staining. D, intracellular triglyceride content. E, Western blotting analysis of SREBP1 and FAS. F–I, NCTC1469 cells were grown in normal medium without oleic acid/palmitic acid and were transfected with the miR-291b-3p mimic or NC for 48 h. F, miR-291b-3p expression measured by real-time PCR. G, lipid accumulation revealed by Oil red O staining. H, cellular triglyceride content. I, levels of SREBP-1 and FAS expression. n = 3 independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001.