FIGURE 6.

Relative mRNA levels of selected genes involved in cholesterol metabolism in the livers of WT and Tm6sf2^−/− mice. A and B, quantitative real-time PCR assays were performed to assay the relative levels of selected mRNA levels in livers of the mice described in the legends to Figs. 3A and 5A. Expression levels were normalized to levels of 36B4 and expressed relative to levels of WT transcript. Values are means ± S.E. (error bars). The official gene symbols were used for all the genes with the following exceptions: AOX, ACOX1; MCAD, ACADM; FPPS, FDPS; SQS, FDFT1; SC4MOL, MSMO1. HMGCR, HMG-CoA reductase; HMGCS1, HMG-CoA synthase. *, p < 0.05; ***, p < 0.001; ****, p < 0.0001.