FIGURE 7.

Intestinal accumulation and absorption of lipid in Tm6sf2^−/− mice. A, jejunal sections prepared from male mice (18 weeks old) fed a high fat diet for 12 weeks were stained with Oil Red O and visualized using a Leica microscope (DM2000) at ×10 and ×63 (inset) magnification as described under “Experimental Procedures.” B, chow-fed male mice (n = 4 mice/group, 16 weeks old) were fasted for 16 h and then gavaged with corn oil (10 μl/g). Blood was collected at the indicated times after gavage, and plasma levels of TG were measured by enzymatic assay. The experiment was repeated, and the results were similar. C, chow fed female mice (n = 6 mice/group, 12 weeks old) were fasted for 16 h prior to the injection of Triton WR-1339 (500 mg/kg) into the tail vein. A total of 30 min after the injection, mice were gavaged with corn oil (50 μl) supplemented with 1 μCi of [¹⁴C]cholesterol (50.8 mCi/mmol) and 5 μCi of ³H-labeled oleic acid (54.5 Ci/mmol). Blood was collected at the indicated times before and after injection of Triton WR-1339, and the radioactivity in the plasma was measured by scintillation counting at the indicated time points. *, p < 0.05. Values are means ± S.E. (error bars).