FIGURE 9.

PDGFA contributes to maintain stemness marker expression in GSG. A, immunofluorescence staining showing NANOG, SOX1, and SHH expression in TG1, TG6, and GB8 cells. Cells cultured in defined medium were transfected with either a non-relevant siRNA (siLuc) or a PDGFA-specific siRNA (siPDGFA). Nuclei were stained with DAPI. Scale bars are shown. B–D, quantification of cells positive for NANOG, SOX1, and SHH staining, respectively. Five independent fields were counted. *, p < 0.05; **, p < 0.001; ***, p < 0.0001; Student's t test. Error bars, S.E.