Depletion of USP10 in A549 cells confers MNNG and 6-TG resistance.
A, knockdown of USP10 increases cell viability to MNNG. A549 cells transduced with lentiviruses containing empty vectors, shUSP10–1 or shUSP10–2 were treated with MNNG of the indicated concentration for 2 days and 3 days. MTT assays were performed. B, knockdown of USP10 increases cell viability to 6-TG. A549 cells transduced with lentiviruses containing empty vectors, shUSP10–1 or shUSP10–2 were treated with 6-TG. MTT assays were performed. C, knockdown of USP10 reduces apoptosis upon treatment of MNNG and 6-TG. A549 cells transduced with lentiviruses containing empty vectors, shUSP10–1 or shUSP10–2 were treated with MNNG or 6-TG at indicated times and concentrations. Cells were lysed, and the anti-PARP1 and anti-β-actin Western blotting analyses were performed. For A and B, the error bars stand for a standard error (S.E.). **, denotes p < 0.001 by a two-tailed Student's t test (n = 6).