FIGURE 5.

Overexpression of MSH2 in USP10-knockdown A549 cells restores the 6-TG- and MNNG-mediated growth inhibition and apoptosis. A, A549 stable pools were transduced with vectors, shUSP10–1 and shUSP10–2, and the latter two pools were transfected with HA-MSH2. Then the above five groups of cells were treated with vehicle, 10 μm 6-TG or 10 μm MNNG for 3 days. MTT assays were performed. B, five groups of cells as shown in 5A were treated with vehicle, 30 μm 6-TG, or 30 μm MNNG for 3 days. Cells were then harvested and lysed. The anti-PARP1, anti-MSH2, anti-USP10, and anti-β-actin Western blotting analyses were performed. For A, the error bars stand for S.E. **, denotes p < 0.001 by a two-tailed Student's t test (n = 6).