TABLE 1.

Description of EPRS mutations used in this study

Gene construct	Description of mutation
EPRS stem loop insertion	Insertion of CTGCAGCCACCACGGCCCCCAAGCTTGGGCCGTGGTGGCTGCAG 3′ of CGTAGCGTGC
EPRS uORF1 ΔCUG1	GGGCTGCGG to GGGAAACGG
EPRS uORF1 ΔCUG2	CACCTGAAC to CACAAAAAC
EPRS uORF1 ΔCUG3	CTCCTGGGA to CTCAAAGGA
EPRS uORF2 ΔUUG1	ACGTTGCAT to ACGAAACAT
EPRS uORF3 ΔUUG2	TCCTTGCTT to TCCAAACTT
EPRS uORF1 CUG2 to optimized AUG	CACCTGAAC to ACCATGGAC
EPRS uORF2 UUG1 to optimized AUG	ACGTTGCAT to ACCATGGAT
EPRS uORF2 UGA to UGG	ACCTGAACC to ACCTGGACC