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. 2016 Mar 21;291(20):10836–10846. doi: 10.1074/jbc.M115.698779

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — The expression of NAD⁺ salvage enzymes and NAD⁺-consuming enzymes in hSOD1^G93A astrocytes is not altered. A, mRNA levels of the indicated genes in confluent non-transgenic (NonTG) and hSOD1^G93A (G93A) spinal cord astrocyte cultures. Each data bar represents the mean ± S.D. (error bars) of at least three independent experiments. B, SIRT1 and SIRT3 protein levels in non-transgenic and hSOD1^G93A astrocytes. Actin levels were used as a loading control. Each lane represents an independent biological replicate. (SIRT1 quantification: non-transgenic, 100 ± 19; hSOD1^G93A, 102 ± 11; SIRT3 quantification: non-transgenic: 100 ± 6; hSOD1^G93A, 92 ± 7. No significant changes were found.)