TABLE 1.
Kinetic folding parameters of XD
D, I, and N indicate denatured, intermediate and native states.
| kDIa | kIDa | kINa | kNIa | kDNa | kNDa | ΔGDI | ΔGIN | |
|---|---|---|---|---|---|---|---|---|
| s−1 | s−1 | s−1 | s−1 | s−1 | s−1 | kcal mol−1 | kcal mol−1 | |
| On pathway | 5700 ± 500 (0.2 ± 0.05) | 2.9 ± 0.7 (0.8 ± 0.05) | 480 ± 70 (0.04 ± 0.05) | 94 ± 10 (0.2 ± 0.03) | 4.2 ± 0.9 (1.0 ± 0.07) | 0.9 ± 0.1 (0.24 ± 0.07) | ||
| Off pathway | 1400 ± 300 (0.3 ± 0.05) | 683 ± 60 (0.16 ± 0.05) | 4700 ± 900 (0.5 ± 0.1) | 39 ± 0.01 (0.25 ± 0.05) | 0.4 ± 0.1 (0.46 ± 0.07) | 2.3 ± 0.5 (0.30 ± 0.15) |
a The microscopic rate constants were calculated by globally fitting the two observed relaxation rate constants to the analytical solution of a three-state mechanism involving an off-pathway intermediate. The m value associated to each rate constant, expressed in kcal mol−1·m−1, is reported in parentheses.