FIGURE 4.
N-terminal conserved region of AtLEA4-5 is necessary and sufficient to induce folding to α-helix under the different conditions tested. Far UV CD spectra of AtLEA4-51–77 (A–D) and AtLEA4-578–158 (E–H) under different temperatures (A and E), different TFE (B and F) and glycerol concentrations (C and G), and under 45% PEG 5,000 (D and H). Difference spectra (Δ10–80 °C; Δ90–0% TFE; Δ80–0% glycerol; Δ45–0% PEG) are shown as insets in each graph. These results were reproduced at least four times in autonomous experiments, using three independent purification batches of AtLEA4-51–77 protein and one batch for AtLEA4-578–158 polypeptide.