TABLE 1.

Percentage of helix, strand, and unordered structures in AtLEA4-2 and AtLEA4-5

Secondary structure content in AtLEA4-2 and AtLEA4-5 proteins was obtained by far UV CD spectrometry and calculated with Dichroweb server.

Treatment	AtLEA4-2			AtLEA4-5
	Helix	Strand	Unordered	Helix	Strand	Unordered
	%			%
Aqueous solution	5	33	61	5	34	61
4% TFE	7	32	61	6	33	61
8% TFE	16	25	58	6	32	61
12% TFE	21	23	56	17	23	59
16% TFE	35	15	51	18	25	55
20% TFE	42	15	44	32	17	51
40% TFE	57	9	33	49	13	39
60% TFE	62	9	30	57	8	35
90% TFE	80	3	16	88	2	10
10% glycerol	5	34	60	4	34	61
20% glycerol	8	32	61	7	36	57
30% glycerol	18	22	60	7	34	59
40% glycerol	21	23	57	18	24	57
50% glycerol	33	12	53	18	23	59
60% glycerol	43	14	43	34	15	51
70% glycerol	49	9	41	42	11	48
80% glycerol	54	12	33	46	9	45
45% PEG 5000	37	11	52	39	9	53