FIGURE 1.
An amiCE229D mutant releases larger [3H]glucosamine-labeled peptidoglycan fragments relative to the wild type and no disaccharide. A–C, N. gonorrhoeae strains wild type (MS11) (A), amiCE229D (KX503) (B), and amiCE229D+amiC (JL537) (C) were metabolically pulse-chase-labeled with [3H]glucosamine, and the released peptidoglycan fragments were fractionated by size-exclusion chromatography. Traces are representative of two independent labeling and chromatography analyses. D, chemical structures of potential variants for released PG fragments found within the numbered peaks in A–C and heretofore referenced as compound families 1–4. Pictured chain lengths for compound families 1 and 2 are based upon their presence in whole sacculi, but the released proportions of compounds 1A–1G and 2A–2D are unknown. Compounds 3A and 3B are released at an ∼1:3 ratio.