. 2016 Mar 16;291(20):10916–10933. doi: 10.1074/jbc.M116.715573

TABLE 1.

PG fragments identified in LC-MS of sacculi digests

—, not observed.

PG peptide fragment	Calculated exact mass	Ion	AmiC (1×)		AmiC (5×)		NlpD (5×)		AmiC + NlpD		AmiC_Q316K
PG peptide fragment	Calculated exact mass	Ion	Observed m/z	Observed mass	Observed m/z	Observed mass	Observed m/z	Observed mass	Observed m/z	Observed mass	Observed m/z	Observed mass
Tetra-tetra-tetra	1347.62	[M + 2H]²⁺	674.82	1347.62	674.82	1347.62	—	—	674.82	1347.62	674.82	1347.62
Tetra-tetra	904.41	[M + H]⁺	905.42	904.41	905.42	904.42	—	—	905.42	904.41	905.42	904.41
		[M + 2H]²⁺	453.22	904.42	—	—	—	—	—	—	—	—
Tetra-tri	833.38	[M + H]⁺	834.38	833.38	834.38	833.38	—	—	834.38	833.38	834.38	833.38
		[M + 2H]²⁺	417.70	833.39	—	—	—	—	417.70	833.38	417.70	833.38
Penta	532.25	[M + H]⁺	533.26	532.25	533.26	532.25	—	—	533.26	532.25	533.26	532.25
Tetra	461.21	[M + H]⁺	462.22	461.21	462.22	461.21	—	—	462.22	461.21	462.22	461.21
		[M + 2H]²⁺	—	—	231.61	461.21	—	—	—	—	—	—
Tri	390.18	[M + H]⁺	391.18	390.18	391.18	390.17	—	—	391.18	390.18	391.18	390.18